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You are planning to use PCR to amplify several regions of a piece of DNA. The...

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Biology

You are planning to use PCR to amplify several regions of apiece of DNA. The sequence of your template DNA is provided belowalong with the sequences of all available primers. Determine whereeach of the primers bind and answer the following:

5' AGGGCCAAATGAGATGAGTCAAAAGCTGCCGATAACCGGATAG 3'

3' TCCCGGTTTACTCTACTCAGTTTTCGACGGCTATTGGCCTATC 5'

Primer 1: 5-TTGGCC 3

P2: 5-GTCAAA-3

p3: 5-AACCGG-3

p4: 5-CCGGTT-3

P1 can bind to the bottom strand?

P4 can bind to the molecule at only one location

Which primer pair would you use to amplify a double stranded pcrfragment of any size from this template

Which primer has the lowest melting point

Using the two selected primers and added all of the PCRcomponents to a test tube answer the following as the polymerasechain rxn proceeds

The concentration of dNTPs will increase,decrease, stay thesame, as the rxn proceeds

The concentration of Taq DNA polymerase will increase,decrease,stay the same, as the rxn proceeds

Answer & Explanation Solved by verified expert
4.2 Ratings (919 Votes)
5 AGGGCCAAATGAGATGAGTCAAAAGCTGCCGATAACCGGATAG 3 3 CCGGTT 5 primer1 will bind to the above strand 5GTCAAA 3 primer2 will bind to the below strand 3    See Answer
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