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You are interested in determining the levels of specific mRNA in blood cells compared to brain...

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Biology

You are interested in determining the levels of specific mRNA inblood cells compared to brain cells. Using real-time PCR and equalnumber of cells in each sample, you find that a minimum level ofdetectable fluorescence is reached after 20 cycles of amplificationstarting with RNA from blood cells and in 10 cycles starting withRNA from brain cells. How would you interpret this result?

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3.9 Ratings (631 Votes)
as we know that realtime PCR is used to determine the gene expression in particular samples here we have brain and blood cells hence we take equal number of cells and then we check how many cycles we need to see the    See Answer
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