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You are given the following: water, 10 mM phosphate buffer pH 7.4, EDTA solid (FW 292.25...

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Biology

You are given the following: water, 10 mM phosphate buffer pH7.4, EDTA solid (FW 292.25 g/mol), 0.5 M Tris HCl, 3 M NaCl, OAAsolid (FW 131.10), NADH solid (FW 709.40), and solutions of NaOHand HCl. Calculate and describe how you would prepare of each ofthe following:  

a. 50 mL of 0.5 M EDTA pH 8.0

b. 10 ml solution containing 10 mM Tris-Cl pH 8.0 + 100 mMNaCl

c. 10 mL of 20 mM OAA dissolved in 10 mM phosphate buffer pH7.4

d. 10 mL of 2 mM NADH dissolved in 10mM phosphate buffer pH7.4

Answer & Explanation Solved by verified expert
3.7 Ratings (482 Votes)
a Required volume required concentration molecular weight of EDTA 0050529225 731 So we will add 731gm of EDTA in 30ml of distilled water Then adjust the pH with NaOH to pH 80 b    See Answer
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