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You are given a sample that has 1.3 mg/ml of purified protein. The molecular weight is...

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Chemistry

You are given a sample that has1.3 mg/ml of purified protein. The molecular weight is 37,000Daltons.

a) the absorbance in a 1cm cuvette is 0.93. calculate the molarextinction coefficient. Be sure to express your answer with correctunits.

b)also calculate the extinction coefficient for concentrationunits of mg/ml, i.e. with units of cm-1(mg/ml)-1

c) The sample is actually contaminated with 0.01 mg/ml DNA, butthe protein concentration, 1.3 mg/ml is correct. explain how thecontaminating DNA will affect your estimate of the extinctioncoefficient?

d) suggest a way in chich you could salvage this situationwithout doing any more experiments. that is, explain how you couldestimate the extinction coefficient in spite of teh DNAcontamination. what key inormation would you need to look up on theinternet or in the library? carefully explain how you wouldcalculate the extinction coefficient.

this is what I have done so far:

a) A=CLε

ε=0.93/(1.3mg/ml)*1cm

=.715(mg/ml)-1Cm-1

=.715(cm-1)(ml/mg)(1L/1000ml)(1000mg/g)(37000g/mol)=26469(L/mol)=26469M-1cm-1

b)26469M-1cm-1=26469cm-1(L/mol)(1000ml/L)(1mol/37000g)(1g/1000mg)=.715(ml/mg)cm-1=.715(mg/ml)-1cm-1

c) this would increase the absorbance, so it would make theextinction coefficient too high

d) ?

Answer & Explanation Solved by verified expert
3.8 Ratings (464 Votes)
D we can perform perform A260280 absorbance using nano drop for a pure DNA sample it is around 18 to 19 if the there is any protein in it the absorbance will be less than 18 280nm Absorbance Method LabLink Most    See Answer
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