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You are given 5mL of 3mg/mL protein solution. You are asked to prepare samples for SDS-PAGE...

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Biology

You are given 5mL of 3mg/mL protein solution. You are asked toprepare samples for SDS-PAGE containing 250ng, 500ng, 750ng, and1000ng. Your pipettes are calibrated to 2uL at the lower limit.Your total volume is 20uL, with 5uL being your loading buffer. Howwould you prepare these samples?

Answer & Explanation Solved by verified expert
4.1 Ratings (527 Votes)
To make these solutions first dilute the given stock Given 3mg in 1ml it means 3000ug in 1000ul so make a solution of 667ug per ml that can be used as stock 3000ug 1000ul so 667    See Answer
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