You are developing primers for a wildlife forensic case and want to identify both species and...

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Biology

You are developing primers for a wildlife forensic caseand want to identify both species and distinguish betweenindividuals.
a) For which type of analysis would you want to develop/usedegenerate primers? Why?
b) If the primer you developed for species ID is 14bp,the mitochondrial genome is 18500 bp, and the nuclear genome is3.2x109bp, how many times would you expect it to bind to each ofthe respective genomes assuming the same primer could bind to boththe mtDNA and nDNA genomes? (2marks)
c) Comment on the specificity of the primer if thisprimer was intended to amplify only a mtDNA region and not a nDNAregion.

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3.9 Ratings (493 Votes)

a For genotypic analysis degenerate primers should be used Degenerate primers are the mixture of similar but unidentical primers that can amplify a same gene from different species If we dont know the exact genetic makeup of wildlife then we must use See Answer

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