You are a researcher in a biochemistry lab which investigates a novel, globular protein production by...

50.1K

Verified Solution

Question

Biology

You are a researcher in a biochemistry lab which investigates anovel, globular protein production by yeast. The director of thelab asks you to produce and purify the protein. Unfortunately, youcould not obtain any information about if the protein isextracellular or intracellular. Within this scope, please propose amethod/methods for isolating the protein after the productionprocess. You have a well-equipped laboratory with the equipmentwould require to isolate and purify. In addition describe anexperiment or set of experiments to prove (or disprove) the proteinis composed of more than one subunit.

Answer & Explanation Solved by verified expert

3.9 Ratings (546 Votes)

Answer Methods of protein Purification The ways utilized in protein purification will roughly be divided into analytical and propaedeutic ways the excellence isnt precise however the deciding issue is that the quantity of protein which will much be pure there upon technique Analytical ways aim to sight and determine a macromolecule in an exceedingly mixture whereas propaedeutic ways aim to provide giant quantities of the macromolecule for alternative functions like structural biology or industrial use In general the propaedeutic ways may be utilized in analytical applications however not the opposite approach around It is four technique for purificationisolation of protein 1 Extraction technique once this extraction method soluble macromolecule are going to be within the solvent and may be separated from cell membranes DNA etc by activity The extraction method additionally extracts proteases which is able to begin digesting the proteins within the answer If the macromolecule is sensitive to chemical change its sometimes fascinating to proceed quickly and keep the extract cooled to weigh down chemical change 2 Precipitation and Differential solubilisation In bulk protein purification a standard commencement to isolate proteins is precipitation with ammonia salt NH42SO4 this can be performed by adding increasing amounts of ammonia salt and assembling the various fractions of precipitate macromolecule One advantage of this technique is that it may be performed inexpensively with terribly See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member