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You are a graduate student studying an inducible transcription factor called X. You make a cellular...

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Biology

You are a graduate student studying an inducible transcriptionfactor called X. You make a cellular lysate and carry out a seriesof centrifugation steps at increasing speeds to localize X. Youfind that prior to induction, X is the supernatant following afourth, very high speed ultracentrifugation step; however, afterinduction it is located in the pellet after only one relatively lowspeed ultracentrifugation step. What might this tell you about howX is regulated? What other techniques might you carry out toconfirm your results?

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4.4 Ratings (771 Votes)
This indicates that X is present in the cytoplasm before induction and localises to the nucleus upon induction to carry out the transcription of its target genes Reason ultracentrifugation is a process of applying various able    See Answer
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