wild type allele TSS gap Wild type expression Agouti O dominant negative O copy number variant O loss of function O gain of function Question 201 agouti viable yellow allele TSS Ectopic expression TSS IAP 99999 unmethylated CG sites The Agouti gene is a determinant of coat color in furry mammals such mice The wild type allele is expressed in the skin in a pattern that leads to the production agouti fur left drawing above The viable yellow allele has an insertion IAP upstream of the normal transcription start site TSS Depending on the methylation status of multiple CG dinucleotides within IAP this insertion can initiate transcription from an abnormal location near the insertion site with transcription reading through the Agouti gene at an elevated level and causing the production of yellow fur right drawing above Drawing adapted from Paro et al Introduction to Epigenetics Springer 2021 This is background information for questions 18 through 23 Wild type expression Agouti Yellow Which of the following classifiers of mutation type would you assign to the viable yellow allele with unmethylated CG sites in IAP 3 pts What fur color do you expect to observe in a heterozygote that has one wild type allele and one viable yellow allele with unmethylated CG sites in IAP