: Why is it necessary to return the tubes to ice after heat-shocking?) (1 mark)(a)...

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: Why is it necessary to return the tubes to ice after heat-shocking?) (1 mark)(a) The tubes need to be kept on ice to prevent protein degradation.(b) The plasmids are unstable upon entering the bacteria and need to be kept cold toprevent it moving around.(c) Placing the tubes on ice closes the pores created by heat-shocking on the bacterial cellwall.(d) DNA will denature if kept at high temperature. Returning the tubes on ice will stabilizeDNA.

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