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Why is it necessary to find the new vector plasmid concentration after undergoing digestion and the...

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Biology

Why is it necessary to find the new vector plasmid concentrationafter undergoing digestion and the clean up?

During the digest clean up what buffers are used and what is thepurpose of each one?

Answer & Explanation Solved by verified expert
3.6 Ratings (527 Votes)
Maintaining perfect vector insert ratio is an essential step for sucessful coloning of gene of interest Normally for sucessful ligation in cloning experiments vector insert ration is maintained as 13 respectively The vector and gene of interest    See Answer
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