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Why is it important to avoid air bubbles when you pack columns for column chromatography? If...

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Chemistry

Why is it important to avoid air bubbles when you pack columnsfor column chromatography? If you accidently introduce air bubblesinto the columns, what are the strategies you could take to removethe air bubbles? When running the column, is it okay to run theliquid below the column level? Why?

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Solution Column chromatography purification method often use gravityflow to process sample solutions through a resin bed that is packed into a column In column chromatography the efficient separation of different components in a mixture depends on efficient and uniform contact of solution with the solid support or resin bed If air bubbles form in the resin bed they limit contact with the resin and impede flow through the    See Answer
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