While perusing the scientific literature late one night, you come across a report that a protein...

90.2K

Verified Solution

Question

Biology

While perusing the scientific literature late one night, youcome across a report that a protein called Thingamajig is thoughtto be involved in the development of antibiotic resistance, anemerging global health crisis. This putative involvement appears toinvolve an interaction with another protein, Whosiewhatsit. Youwould like to study Thingamajig, and its interaction withWhosiewhatsit, but neither protein has been cloned.
a) How would you set out to study these proteins and theirinteraction? Describe in general terms (a flowchart, for example),filling in the details once this framework is assembled. To get youstarted, how would you obtain the information necessary to expressand purify these proteins, and how would you manipulate thisinformation?
b) Assuming Thingamajig is positively charged and Whosiewhatsitis negatively charged, what purification approach might you use topurify each of them independently? What approach might you take topurify the complex of the two?
c) What approach would you take to identify the amino acidresidues â€” in both Thingamajig and Whosiewhatsit â€” that underlietheir interaction with one another?

Answer & Explanation Solved by verified expert

4.4 Ratings (607 Votes)

A The best ways to detect these two proteins interaction are Coimmunoprecipitation It can confirm interactions using a whole cell extract where proteins are present in their native form in a complex mixture of cellular components Tandem affinity purificationmass spectroscopy TAPMS TAPMS is based on the double tagging of the protein of interest on its chromosomal locus followed by a See Answer

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member