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Which statement is false regarding DNA/RNA isolation? a) Remove protein: proteinase K, pronase, phenol/CHCl3 b) Concentrate with...

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Biology

Which statement is false regarding DNA/RNA isolation?

a) Remove protein: proteinase K, pronase, phenol/CHCl3

b) Concentrate with EtOH + salt

c) DNA: DNAase denatured by heat (650C), EDTA

d) RNA: RNAase – very unstable, need to inhibit RNAases(cellular or extraneous)

Answer & Explanation Solved by verified expert
4.4 Ratings (904 Votes)
Nucleic acid DNA or RNA is the genetic material of all organisms It is DNA in majority of organisms For cutting the DNA with restriction enzymes it needs to be pure and free from other macromolecules Because the DNA is covered by the membranes it has to break the cell open to release DNA and other macromolecules like RNA proteins    See Answer
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