Which of the following is INCORRECT whenconsidering how to identify the successful incorporation of a DNAinsert into a vector?
A. Colony PCR indicates the presence and size of an insert
B Ampicillin resistance indicates the presence of an insert
C Blue/white screening indicates the presence of an insert
D DNA sequencing confirms the identity of a correct insert
A mutation in the iron response element (IRE)-binding proteincauses it to have a much higher affinity for the IRE compared toiron. Predict the most likely outcome of this mutation:
A Cellular iron levels will rapidly increase
B Transcription will be blocked if the IRE is located upstreamof the protein-coding region
C Translation of all IRE-containing transcripts will beinhibited
D Translation of iron storage proteins will proceed via a 5'cap-independent pathway
A matrix protein, ELN, is highly expressed in skin cells, butnot expressed at all in liver cells. The ELN protein is encoded bythe eln gene. Which of the following most likely accountsfor tissue-specific ELN expression?
A The eln gene has a stronger promoter in skin cells than inliver cells
B A transcription factor needed for ELN expression may bemissing in liver cells
C The RNA polymerase II may be mutated in liver cells
D The eln gene may lack an enhancer region in liver cells
