Which of the following enzymes could be used to excise most of the gene for subunit...

Free

60.1K

Verified Solution

Question

Biology

Which of the following enzymes could be used to excise most ofthe gene for subunit 1 of cytochrome c oxidase without fragmentingany of the other genes? [Hint: use the 2 cutters displayoption.]
BstEII
SgrAI
BglI
AclI

Answer & Explanation Solved by verified expert

3.8 Ratings (555 Votes)

P.denitrificans cyc A and cta DII genes for cytochrome c 550 and cytochrome c oxidase beta subunit I sequence was take from NCBI.

GTCGACATGGGCCTGCCGGTCGGCATGGATGACCTGGACCTGGCCCTGCGCCGCAGCTTCGCACGGAACT
					TTCCGCCGCTCGCCGGCTGATTTTCGCCCTGCGACATATTCTCCCTCAGCCGTTCTACGACGTTTTTCCT
					GTATTCTGCCCGCTTGGCACATGATAGCCCTGTCAATCGGGAAGCGGAGAGGTCCGTTGCCCGGACATGA
					GCGATACCCAAGAGGAAACGCGATGAAGATCAGCATCTATGCCACTCTCGCCGCCATCACCCTCGCCCTG
					CCCGCTGCGGCCCAGGATGGCGACGCCGCCAAAGGCGAGAAAGAATTCAACAAGTGCAAGGCTTGCCACA
					TGATCCAGGCGCCGGACGGCACCGACATCATCAAGGGCGGCAAGACCGGGCCCAACCTTTACGGCGTCGT
					CGGCCGCAAGATCGCCTCGGAGGAGGGCTTCAAATACGGCGAAGGCATCCTCGAGGTCGCCGAAAAGAAC
					CCCGACCTGACCTGGACCGAGGCCGACCTGATCGAATACGTCACCGACCCCAAGCCCTGGCTGGTCAAGA
					TGACCGACGACAAGGGCGCCAAGACCAAGATGACCTTCAAGATGGGCAAGAACCAGGCCGACGTGGTGGC
					CTTCCTGGCCCAGAACTCGCCCGATGCGGGCGGCGACGGCGAGGCTGCGGCCGAGGGCGAATCGAACTGA
					TCCCGACGCCTCCGAGATGTCTCGACGGCCCCGCCCCTGCGTGCGACAAAAGCGCGCAGGGGCGGACTTT
					TAGTCCTTTCTTCGCATTGCGAGGGGTAGGATTGTGGTCTAGAAACAGGCGAGTCCGTCGGCCTTTGCGC
					CGGCCAGCCGCATGTCTAGGGAGTCCACGCATGGCAGACGCAGCCGTTCACGGCCACGGTGACCATCATG
					ACACCCGCGGGTTCTTCACCCGCTGGTTCATGTCAACAAACCACAAGGATATCGGTATCCTTTACCTGTT
					CACGGCCGGCATCGTCGGCCTGATCTCGGTATGCTTCACCGTCTATATGCGGATGGAACTGCAGCATCCG
					GGCGTGCAATACATGTGCCTGGAAGGCGCGCGTCTCATCGCCGACGCCTCGGCGGAATGCACCCCGAACG
					GACACCTGTGGAACGTCATGATCACCTACCACGGCGTGCTCATGATGTTCTTCGTCGTGATCCCGGCACT
					GTTCGGCGGTTTCGGCAACTATTTCATGCCGCTGCATATCGGCGCCCCGGACATGGCCTTCCCGCGGCTG
					AACAACCTCTCCTACTGGATGTATGTCTGCGGCGTGGCCCTGGGCGTCGCCTCGCTGCTGGCGCCGGGCG
					GCAACGACCAGATGGGTTCGGGCGTCGGCTGGGTGCTCTACCCGCCGCTCTCGACCACCGAGGCGGGCTA
					TTCCATGGACCTGGCGATCTTTGCCGTCCACGTCTCGGGTGCCTCGTCGATCCTGGGCGCGATCAACATC
					ATCACCACCTTCCTCAACATGCGCGCACCGGGCATGACGCTGTTCAAGGTGCCGCTGTTTGCCTGGTCGG
					TCTTCATCACCGCCTGGCTGATCCTGCTGTCGCTGCCGGTTCTGGCGGGCGCGATCACCATGCTGCTGAT
					GGACCGCAACTTCGGCACGCAGTTCTTCGATCCGGCCGGCGGCGGCGACCCGGTGCTTTACCAGCACATC
					CTGTGGTTCTTCGGCCATCCCGAGGTCTATATCATCATCCTGCCGGGCTTCGGCATCATCAGCCACGTCA
					TCTCGACCTTCGCCAAGAAGCCGATCTTCGGCTACCTGCCGATGGTGCTGGCCATGGCGGCGATCGGCAT
					CCTGGGCTTCGTCGTCTGGGCGCACCACATGTACACGGCCGGCATGTCGCTGACCCAGCAGGCCTATTTC
					ATGCTGGCGACCATGACCATCGCGGTGCCCACCGGCATCAAGGTCTTCTCGTGGATCGCGACCATGTGGG
					GCGGCAGCATCGAGTTCAAGACGCCGATGCTCTGGGCCTTCGGCTTCCTGTTCCTGTTCACCGTCGGCGG
					CGTGACCGGCGTGGTGCTGAGCCAGGCGCCGCTGGACCGGGTCTATCACGACACCTATTACGTCGTGGCC
					CACTTCCACTACGTGATGTCGCTGGGCGCGGTGTTCGGCATCTTCGCCGGGGTCTATTACTGGATCGGCA
					AGATGTCGGGCCGGCAATACCCGGAATGGGCGGGCCAGCTGCATTTCTGGATGATGTTCATCGGCTCGAA
					CCTGATCTTCTTCCCGCAGCACTTCCTGGGCCGCCAGGGCATGCCGCGGCGCTATATCGACTATCCGGTC
					GAGTTCGCCTATTGGAACAACATCTCGTCGATCGGCGCCTATATCTCCTTCGCGTCCTTCCTGTTCTTCA
					TCGGCATCGTGTTCTACACGCTCTTCGCCGGCAAGCGCGTGAACGTGCCGAACTACTGGAACGAGCATGC
					CGACACGCTGGAATGGACCCTGCCCTCGCCGCCGCCCGAGCATACCTTCGAGACCCTGCCCAAGCGCGAG
					GACTGGGATCGCGCACACGCGCATTGATCCCCGCATTGATCCAACGATGCCCTATGCATGGCATGATGCG
					GCCCCGGAGAATTCCGGGGCCGCATGCTTTCGGCTCGAG

by using http://www.restrictionmapper.org/ analysis were done:-

BglI - Positions are 154, 635, 1073, 1807, 2276

BstEII - Position is 898

SgrAI, AclI are non cutter.

SO, Answers are BstEII, BglI.

Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:

Unlimited Question Access with detailed Answers
Zin AI - 3 Million Words
10 Dall-E 3 Images
20 Plot Generations
Conversation with Dialogue Memory
No Ads, Ever!
Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!

Become a Member