Which of the following components of urease media is added for the differentiation of rapid and...

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Biology

Which of the following components of urease media is added forthe differentiation of rapid and slow urease producers?
Multiple Choice
Urease
Yeast extract
Phenol red
Phosphate buffer
Which of the following classes of bacteria are being tested forusing the urease test?
Multiple Choice
Gram-negative enterics
Gram-positive streptococci
Gram-positive staphylococci
Gram-negative non-enterics
Which of the following metabolic waste products would be aresult of nitrate reductase?
Multiple Choice
Nitrite
Nitrous oxide
Atmospheric nitrogen gas
Ammonium
Nitrite reductase is produced by bacteria that carry outdenitrification in the nitrogen cycle.
True or False
Bacteria positive for nitrite production will remain colorlessafter the addition of nitrate reagents A and B and HCl.
True or False

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4.1 Ratings (784 Votes)

Answer 1 The correct option is third Phenol Red Urease test is commonly used for differentiating enteric bacteria These bacteria hydrolyses urea and produce CO2 and ammonia Presence of ammonia shifts the pH of the medium towards alkalinity which is detected by PHENOL RED Color of phenol red at near neutral pH is orange and alkalinity causes color shifting from orange to Pink and this is used as an indicator for differentiation of rapid and slow urease producers Option first is incorrect urease enzyme catalyzes the hydrolysis of urea however they do not give directly any indicator for differentiation See Answer

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