When researches incubate whole red blood cells with lactoperoxidase, hydrogen peroxide and radioactive iodine I 125...

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Biology

When researches incubate whole red blood cells withlactoperoxidase, hydrogen peroxide and radioactive iodine I 125 andthen separate the proteins in SDS gel, several radioactive linesare obtained cause iodine binds to prominent tyrosine and histidineresidues outside the cell membrane.
When researches incubate whole red blood cells with galactoseoxidase and reduce them with sodium borohydride that containstritium (H-BH43) several radioactive lines are obtained which arejust some of the proteins that have been marked with radioactiveiodine , thus galactose residues stand out of the cell.Â
a) Explain the difference in the number of proteins obtained in thetwo experiments above
b) Why the results of the second experiment do not reflect mark ofall the sugars in the membrane?Â
c) What results will be obtained if we conduct an experimentidentical to that described in the question but with inversevesicles (these are closed vesicles containing all membranecomponents but where the outer leaf of the membrane turns inwardand inward leaf outward)?

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4.4 Ratings (809 Votes)

a In the first experiment RBC even after the addition of lactoperoxidase and hydrogen peroxide and radioactive iodine 95 of the cells remain intact but after the experiment there is a reduction of good cellsproteins which are See Answer

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