What result would you obtain if you carried out the incubation with an anti-GFP antibody instead...

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Biology

What result would you obtain if you carried out the incubationwith an anti-GFP antibody instead of anti-RFPantibody as the primary antibody? Explain why.

What result would you obtain if you switched the antibodies?Meaning the primary antibody was used as the secondary antibody andvice versa. Explain what is happening on the blot and what isdetected.

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Fluorescent proteins are unique large 25 KD proteins with the property of bioluminescence The first fluorescent protein to be discovered was the green fluorescent protein GFP naturally present in the jellyfish Aequoria victoria way back in 1962 Most of the other fluorescent proteins emitting different colours including the red fluorescent protein or RFP are derived from GFP through mutations However there also exist naturally occuring RFP such as the DsRED2 RFP obtained from the red coral Discoma sp The basic structure of    See Answer
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