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what procedure allows us to isolate and extract plasmid DNA and to avoid isolating the larger...

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Biology

what procedure allows us to isolate and extract plasmid DNA andto avoid isolating the larger circulargenomic-chromosomal DNA?

Why do many restriction enzymes require a 37 C temperature to beactive and to hydrolyze DNA molecules while other restrictionenzymes require 50 C temperature to be active and to hydrolyze DNAmolecules?

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Plasmid DNA is very frequently isolated by alkaline lysis method In alkaline lysis method the plasmid containing cells pellet are resuspended in EDTA containing buffer The EDTA chelates the divalent cations and weekend the wall of cells Further the cells are lysed by adding lysis    See Answer
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