What kinds of materials obtained from a crime scene mightcontain DNA? (2 pts)
Consider the following DNA molecule
5ʹCCTTGGGGCCAATTGGCCGTACCGAATTCGCCGAATTCCGGAATTGGCCTACGGGCTCGGGCCGG3ʹ
3ʹGGAACCCCGGTTAACCGGCATGGCTTAAGCGGCTTAAGGCCTTAACCGGATGCCCGAGCCCGGCC5ʹ
How many bp is the original fragment?(1 point)
The enzyme HaeIII has thefollowing restriction site   5ʹ GGË…CC 3ʹ        Â
                                                                               3ʹ CC˄GG 5ʹ
If digested with HaeIII, howmany fragments are formed if the DNA is linear? (1point)
If digested with HaeIII, howmany fragments are formed if the DNA is circular? (1point)
Consider the following DNA molecule
5ʹATTCGCGAATTCGGTACCGAATTGGCAGATTCGCCGAATTCCCGTACGGAATTAGTTAAC 3ʹ
3ʹTAAGCGCTTAAGCCATGGCTTAACCGTCTAAGCGGCTTAAGGGCATGCCTTAATCAATTG 5ʹ
How many bp is the original fragment?(1 point)
(Recognition site for EcoRIis given previously)
If digested with EcoRI, howmany fragments are formed if the DNA is linear? (1point)
If digested with EcoRI, howmany fragments are formed if the DNA is circular? (1point)
Using the results from question #2 (for linear DNA), draw theresults on a gel( “—“ = the well). Well A contains the complete,intact, undigested sample/sequence; well B contains digestedsample/sequence. (4 points)
AÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â B