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What is the split gal4 system? Why is it needed to label some cell types? Describe...

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Medical Sciences

What is the split gal4 system? Why is it needed to label somecell types? Describe an experiment that combines split gal4 withscRNA-seq to precisely define cell identities in the drosophilabrain. Describe the protocol of the experiment in detail.

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The GAL4UAS system is a biochemical methodused to study gene expression and function in organisms such as thefruit fly It has also been adapted to study receptorchemicalbinding functions in vitro in cell culture It isconsidered a powerful technique for studying the expression ofgenesThe system has two parts the Gal4 gene encoding the yeasttranscription activator protein Gal4 and the    See Answer
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