What is the purpose of heat fixing a slide? List the 4 major steps of the...

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Medical Sciences

What is the purpose of heat fixing a slide?
List the 4 major steps of the gram stain and explain what ishappening to both Gram positive and Gram negative cells in eachstep.
Why donâ€™t we heat fix in the capsule stain?
What are the major things that are done in the acid fast stainprocedure that allow acid fast cells to be stained?
Hypothetically, let's say you perform an endospore stain andobserve that the endospores formed in your specimen exhibit aspherical and subterminal morphology. Why is it useful knowthis?

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1 Heat fixing the slideHeat fixing kills the bacteria and makes them fixed to the glass slide so that it they are not washed away while doing the staining It also makes them take the stain readily 2 The principle of Gram staining Gram positive cells have a more acidic protoplasm which may account for the retaining the basic primary dye more strongly than Gram negative bacteria The peptidoglycan of Gram positive bacteria is thick and thus able to retain the dyeiodine Complex The high lipid content of Gram negative bacteria makes them permeable to secondary dye after See Answer

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