What four components are added to a transmitted light microscope to make it a DIC (differential...

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Biology

  1. What four components are added to a transmitted lightmicroscope to make it a DIC (differential interferencecontrast (DIC) microscopy )microscope?

  2. How do you adjust the bias on a DIC(differential interference contrast (DIC)microscopy ) microscope?

  3. Although the images produced by a DIC(differential interference contrast (DIC)microscopy )microscope appear to bethree-dimensional, they do not actually give real 3D information.Explain why.

  4. Why is it important that all glass and plastic-ware usedon or with the DIC (differential interference contrast(DIC) microscopy )microscope isstrain-free?

  5. What are the advantages of DIC (differentialinterference contrast (DIC) microscopy )microscopy over phase contrast microscopy?

  6. What are the advantages of phase contrast microscopyover DIC (differential interference contrast(DIC) microscopy )microscopy?

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Ans 1 Four basic components areadded to a transmitted light microscope to make it a DIC microscope a Linear Polarizer Inserted into the optical pathway between the microscope light port or anywhere after the illumination source collector lens and the condenser lens assembly this component is designed to produce the necessary planepolarized light for interference imaging The vibration plane transmission axis for the electric vector component is oriented in an EastWest direction right to left when standing in front of the microscope typical of a standard polarized light microscope b Condenser Wollaston or Nomarski Prism In order to separate the polarized light emanating from the polarizer into two components a specialized beamsplitting prism condenser prism is placed in or near the conjugate focal plane of the condenser iris diaphragm aperture    See Answer
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