what form/s of MAPK does the anti-total map kinase antibody detect, and what form/s of MAPK...

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Biology

what form/s of MAPK does the anti-total map kinase antibodydetect, and what form/s of MAPK does the anti-active map kinaseantibody detect?

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Researches suggest that Mitogenactivated protein kinase MAPK are also known as the extracellular signalregulated kinase ERKs they exist in two isoforms namely ERK1 MAPK3 and ERK2 MAPK112 ERK2 is mapped to human chromosome 22q11213 ERK1 is located on human chromosome 16p11214 AntiMAP Kinase Activated Diphosphorylated ERK12 antibody Mouse monoclonal mouse IgG1 isotype is derived from the MAPKYT hybridoma produced by the fusion of mouse myeloma cells NS1 and splenocytes from BALBc mice immunized with a synthetic phosphorylated peptide containing amino acids corresponding to the phosphorylated form of ERKactivation loop conjugated to KLH The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents Product Number ISO2 Specificity Monoclonal AntiMAP Kinase Activated Diphosphorylated ERK12 is specific for the active duallyphosphorylated form of MAP kinase ERK1 and ERK2 44 kDa and 42 kDa respectively Reactivity has been observed with human bovine rat mouse hamster Xenopus Drosophila Spodoptera frugiperda C elegans and yeast The antibody reacts specifically with the diphosphorylated form of MAP kinase ERK1 and ERK2 It does not recognize the nonphosphorylated or the monophosphorylated forms of MAP kinase or the diphosphorylated forms of JNK and p38 MAP kinase The epitope recognized by the antibody contains the phosphorylated threonine See Answer

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