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What do the terms transmittance and absorbance mean in terms of sample concentration quantification? The BCA assay is...

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Biology

What do the termstransmittance and absorbance mean in terms ofsample concentration quantification?

The BCA assay is a common assay forprotein quantification. Explain how it works for this purpose.

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4.4 Ratings (809 Votes)
Beer Lamberts lawAbsorption of light when it is passed through a sample isdirectly proportional to the concentration path length and themolar absorbtivity of a sampleA bcA Absorbance molar absorbtivity L mol1 cm1 c    See Answer
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