What are the pros and cons of using monoclonal antibodies vs polyclonal antibodies? If you had a...

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Biology

What are the pros and cons of using monoclonal antibodies vspolyclonal antibodies?
If you had a protein expressed at low levels that you weretrying to isolate, which would you use?

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Monoclonal Antibodies These are antibodies that detect a single epitope of an antigen They are produced by Hybridoma technology where an antibody producing B cell from spleen of immunized animal is fused with a myeloma cell line leading to production of larger number of these antibodies Pros Advantages Cons Disadvantages Large number of these identical antibodies can be produced as cell lines are used Monoclonal antibody production takes longer time of around 69 months These antibodies produced show no variations as they are produced by the cell line They are homogenous antibodies As they react only to a single epitope their detection efficiency is low Even small changes in epitope make these antibodies ineffective Hence they See Answer

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