Using the blanks provided, put the steps of involved in mapping a bacterial chromosome by conjugation...

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Biology

Using the blanks provided, put the steps of involved in mappinga bacterial chromosome by conjugation in chronological order usingthe numbers 1-6 in the blanks provided.
a. _ _ Cells that mated are checked for transfer of othergenes from the Hfr to the Fâ€“ strain.
b. _ _ At regular intervals, conjugation is interrupted byshearing the mating bacteria away from each other.
c. _ _ For each time point, cells that mated areselectedâ€”for instance, by requiring that a prototrophy orantibiotic resistance from each parent be present in one cell.
d. _ _ Select a donor Hfr strain and a recipient Fâ€“ strain.The two strains must be different in genotype for several markergenesâ€”for example, leuâ€“ and leu+, azir and azs.
e. _ _ The two strains are mixed together in a nutrientmedium to allow conjugation to begin. After a few minutes, theculture is diluted to prevent new conjugations.
f. _ _ Order and distance of transferred genes aredetermined by comparing the time required for them to transfer fromthe Hfr to the Fâ€“ strain.

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The correct order is d e b c a and finally f d 1 Select a donor Hfr strain and a recipient F strain The two strains must be different in genotype for several marker genesfor See Answer

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