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Using 1.0 nM enzyme X for the enzyme kinetics experiments, you obtained the reaction rates at...

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Chemistry

Using 1.0 nM enzyme X for the enzyme kinetics experiments, youobtained the reaction rates at different concentrations ofsubstrate (shown in the following table). Assume that the enzyme Xfollows a typical Michaelis-Menten kinetics.

[substrate] (μM) Reaction rate (μmol/min)

2 7

5 17

10 29

20 44

50 67

100 80

200 89

500 95

1000 98

a) Estimate the Vmax and Km values of the enzyme in thisreaction using double reciprocal plot.

b) If 5.0 nM of the enzyme X is used in the experiment, whatwill be the Vmax and Km of the enzyme X?.

Answer & Explanation Solved by verified expert
4.3 Ratings (843 Votes)
a So I have taken reciprocal of both data series and plottedthe following1S micromolar1    See Answer
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