Treatment of cells with the BOBA drug induces apoptosis of cells and leads to DNA fragmentation. A)...

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Biology

Treatment of cells with the BOBA drug induces apoptosis of cellsand leads to DNA fragmentation.

A) (4pts) Describe the cellular mechanism that causes DNAfragmentation during apoptosis to occur. (4 sentence max)

B) (8pts) Describe an experiment, step by step, that can be used todetermine how many hours after BOBA treatment DNA fragmentationoccurs. (8 sentence max)

C) (4pts) One of your friends said they would use an Annexin V andPI staining technique to identify how many hours post BOBAtreatment DNA fragmentation occurs. Why wonâ€™t this experimentalmethod work to answer this experimental question? (4 sentencemax)

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3.9 Ratings (495 Votes)

A Cellular mechanism causing DNA fragmentation during apoptosis Apoptosis is programmed cell death The DNA fragmentation is activated by an enzyme caspase activated DNase CAD A protein inhibits CAD which is called as ICAD inhibitor CAD During apoptosis caspase3 cleaves ICAD CAD complex to free the CAD enzyme The CAD cleaves the interlinker sites at the nucleosomes to cleave the chromosomal DNA leading to fragmentation B See Answer

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