Topic: Protein interaction and phase separation (Subject: Functional Genomics and Bioinformatics) Please answer both Part A and...

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Biology

Topic: Protein interaction and phase separation (Subject:Functional Genomics and Bioinformatics)
Please answer both Part A and B if possible, IF NOTPRIORITISE ON PART B
Consider the following scenario. A mutation in gene M wasdiscovered to be a highly penetrant risk factor for disease P.There is no information on what the function of gene M is or whatother proteins the gene product of M interacts with. All that isknown is that M is abundantly and ubiquitously expressed in allcell types.
A. Describe in detail one (1) experimental approach that can beused for defining what proteins bind to gene product M and how themutation alters the binding partners.
B. If purified gene product M were found to phase separate intodroplets in a test tube, describe an experiment that that woulddistinguish whether the protein was in a liquid state or a glassyor fibrillar state.

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4.1 Ratings (793 Votes)

1 To identify what proteins interact with proteinM we can perform several experiments a Coimmunoprecipitation Use a specific antibody against proteinM and pull down all the proteins that are associated with it See Answer

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