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To make 10X gel electrophoresis running buffer, 30.0 g Tris base, 144.0 g glycine, and 10.0...

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Chemistry

To make 10X gel electrophoresis running buffer, 30.0 g Trisbase, 144.0 g glycine, and 10.0 g SDS must be dissolved into 1000mL water. The working stock is a 1X concentration. a. What is theconcentration in % w/v of each of the 3 reagents in the 10X stock?b. Describe how you would make 1 L of a 1X working stock solution.c. If 50 mL of the 1X working stock are used to run a gel, how manygrams of each of the 3 reagents are in the 1X solution?

Answer & Explanation Solved by verified expert
4.3 Ratings (700 Votes)
a Volume of water 1000 ml Mass of water volumedensity 100010 1000 g Total mass of solution mass of watermass of trismass of glycinemass of SDS 10003001440100 11840 g Considering density as 10 gml of solution volume    See Answer
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