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This is a three part question: a) What are some major differences between making transgenic mice by...

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Biology

This is a three part question:

a) What are some major differences between making transgenicmice by microinjection and creating knockout mice by homologousrecombination (before the advent of CRISPR/Cas9 technology)?

b) Explain how negative and positive selection work whencreating a knockout mouse. Why are both types of selectionuseful?

c) Why is the creation of knockout mice so much easier whenusing CRISPR/Cas 9 genome editing?

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