This is a confocal microscopy class. So please answer clear anddetail according to the subject. Thank you

1.Explain how light diffraction causes the lateral point spreadfunction of light as shown by Airy. Illustrate with a diagram ofthe PSF in the lateral direction.

2.What is the relative size of the point spread function in thevertical compared to lateral direction and how does this affect theshape of the voxels we record in a optimal resolution z-stack.

3.Is the point spread function wider or narrower with largeaperture lenses? Explain why.

4.Does a larger aperture lens have higher resolution (moredetail) or lower resolution (poor detail)? Or is aperture sizeunrelated to resolution?

5.Definedynamicrange.Explaintheimportanceofadjustingthedynamicrangeofthe light intensity sensors (PMTs) and how the confocal encodesintensity into numbers.

6.Explain the advantage and disadvantage of frame-averaging.

6b.Explain how to optimize the resolution of your scanned imageto avoid oversampling and minimize file size.

7.Make a list of the most functionally-important parts inside ofthe scan head box and briefly explain what each part does. Hint1:there are 5 kinds of things, and the AOTFs & lasers are not inthe scan head box. Hint2: Without these 5 things, it would not be alaser scanning confocal microscope.

8.Explain why immersion oil is used for some lenses. Explain howit works.

9.Explain the relationship between the point-spread function andsuper-resolution.

10. What is chromatic aberration?

11. How does one detect cross-talk and bleed-through? How canyou avoid it, and how can you correct for it if you could not avoidit?

12. Describe the three main ways to manage autofluorescence.