This 9-year-old boy was taken to the emergency department with a sore throat. On examination, he...

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This 9-year-old boy was taken to the emergency department with asore throat. On examination, he had redness of the throat andslightly swollen glands. The physician assistant ordered a throatculture and blood drawn for an antistreptolysin-O antibody (ASO).An antibiotic was prescribed for a 10-day period. His mother wastold to make an appointment with his pediatrician for a follow-up.At the follow-up visit 2 weeks later, the results of the laboratorytest revealed a throat culture with a few colonies ofÎ²-streptococci. The qualitative ASO test result was reported aspositive. The acute serum was frozen at the time of testing. Thepediatrician ordered a convalescent specimen to be testedsemiquantitatively in parallel with the acute specimen for an ASOtiter.
The results of the parallel testing of the acute and convalescentspecimens revealed the following:
â€¢ Acute specimen positive, 1:1 dilution/titer (IU/mL 200)
â€¢ Convalescent specimen positive, 1:4 dilution/titer (IU/mL800)

Thinking Group Discussion Questions
1. Is the difference between the acute and convalescent titerssignificant?

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1 The ASO test is used to detect group A streptococcal infections although it detects group C and group D infections as well It detects the production of ASO antibodies produced in response to the streptolysin antigen expressed by these streptococci SSO See Answer

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