There is an enzyme amylase used in Bioethanolproduction at large scale...

There is an enzyme â€œamylaseâ€ used in Bioethanol production at large scale. The enzyme has excellent...

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Biology

There is an enzyme â€œamylaseâ€ used in Bioethanolproduction at large scale. The enzyme has excellent Kcat and Kmvalues but there is issue of thermostability. The enzyme cannotwork beyond 50oC temperature which is not optimum for industry. Wehave been giving a target to increase its thermostability by usingRational Design. Can you please let us know that which region(Helix, Sheet, Loops) will be targeted to increase thermostabilityof enzyme and why?

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Ans The amylases are endoacting enzymes that hydrolyze starch by randomly cleaving the 14dglucosidic linkages between the adjacent glucose units in a linear amylose chain They have significant advantages in a wide range of applications particularly in the food industry EfAmy has the characteristics of a psychrophilic amylase such as the highest hydrolytic activity at a low temperature and high thermolability which is the major drawback of coldactive enzymes in industrial applications Applied sitedirected mutagenesis See Answer

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There is an enzyme â€œamylaseâ€ used in Bioethanol production at large scale. The enzyme has excellent...