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The ubiquitin protein from the malaria parasite was treated with chymotrypsin, and the resulting fragments were...

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Biology

The ubiquitin protein from the malaria parasite was treated withchymotrypsin, and the resulting fragments were sequenced. A secondsample of the polypeptide was treated with trypsin, and thefragments were sequenced. What is the sequence of thepolypeptide?

Chymotrypsin fragments:

AGKQLEDGRTLSDY

IPPDQQRLIF

VKTLTGKTITLDVEPSDTEIN-VKAKIQDKEGI

NIQKESTLHLVLRLRGGMQIF

Trypsin Fragments:

LR

AK

EGI

IQDK

LIFAGK

QLEDGR

TLTGK

IPPDQQR

GGMQIFVK

TLSDYNIQK

ESTLHLVLR

Answer & Explanation Solved by verified expert
3.9 Ratings (760 Votes)
The sequence is IPPDQQRLIF AGKQLEDGRTLSDY NIQKESTLHLVLRLRGGMQIF VKTLTGKTITLDVEPSDTEINVKAKIQDKEGI Chymotrypsin cleavage site is NHRRCO R phenylalanine F Tyrosine Y tryptophan W R is not proline P The cleavage site of trypsin is NHRCO R Lysine K or arginine R In both    See Answer
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