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The restriction endonuclease BamH1 has the recognition sequence G/GATCC and Sau3A the recognition sequence /GATC. Genomic...

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Biology

The restriction endonuclease BamH1 has the recognition sequenceG/GATCC and Sau3A the recognition sequence /GATC. Genomic DNA (G/Ccontent = 56%) is digested with Sau3A and ligated into a vectordigested with BamH1. What percentage of fragments could potentiallybe ligated into the vector and what is the average fragment lengthof the insert DNA?

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Ans As given in the question that BamH1 has the recognitionsequence 5GGATCC3 and its complementary sequence ispalindromic to the original site and Sau3A has the recognitionsequence 5NGATC3 where N can be any nucleotide and itscomplementary sequence is also    See Answer
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