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The regulation of aspartate derived amino acids in Arabidopsis thaliana is depicted as an integrated network...

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Biology

  1. The regulation of aspartate derived amino acids in Arabidopsisthaliana is depicted as an integrated network involving pathway endproducts that act as allosteric effectors on enzymes inintermediate steps.

    1. Explain the downstream consequence of a reduction in the amountof S-Adenosyl methionine (SAM) on the overall flux of carbonthrough the various branches of this metabolic pathway.

    2. The first step in converting aspartate into other amino acids isthe phosphorylation of aspartate. In A. thaliana, there are fivedistinct aspartate kinase enzymes, two of which (AK I and AK II)are bi-functional enzymes that also possess homoserinedehydrogenase activity (HSDH I and
      HSDH II, respectively). What is the net effect of combining AK andHSDH activities into one enzyme? How does regulation of AK I/HSDH Iand AK II/HSDH II impact the flow of carbon skeletons toward thepossible end products in this pathway?

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