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The primary sequence of the peptide listed below. H2N -(1) - (2) - (3) - (4)...

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Biology

The primary sequence of the peptide listed below.

H2N -(1) - (2) - (3) - (4) - COOH

H2N - Asp-Thr-Phe-Lys-COOH

H2N – D -T – F – K – COOH

Use the one letter amino acid code and identify from N-terminusto C-terminus.

a.Determine the pI of the peptide. (Round to 2 decimalpoints)

b. Would mutating residue #3 to Ala change the pI of thepeptide? Explain.

c. Which type of noncovalent interaction would the indicatedresidue side chain make at the given pH? (H-bond, Hydrophobic orVan der Waals, or Ionic bond or Salt bridge)

Residue 1 @ pH 5.0

Residue 2 @ pH 6.0

Residue 3 @ pH 11.0

Residue 4 @ pH 10.0

d. Calculate the fraction of the peptides C-terminus protonatedat pH 2.5 (Round to 2 decimal points)

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