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The Klenow fragment is produced when DNA polymerase 1 from E.coli is enzymatically cleaved by the...

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Biology

The Klenow fragment is produced when DNA polymerase 1 fromE.coli is enzymatically cleaved by the protease subtilisin. Usingdiagrams, explain what the Klenow fragment is and why the fragmentis better suited for site-directed mutagenesis than intact DNApolymerase 1?

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Ans The Klenow Fragment is a cleavage product of the Ecoli DNA Polymerase I with the protease subtilisin It lacksthe 53 exonuclease activity of DNA Pol I a feature that makesthe enzyme unsuitable for    See Answer
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