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The herbicide 2,4-D is being analyzed in plant matter using triphenylphosphate (TPP) as an internal standard....

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Chemistry

The herbicide 2,4-D is being analyzed in plant matter usingtriphenylphosphate (TPP) as an internal standard. A calibrationsample is prepared by pipetting 10.0 µL of 119 µg/mL 2,4-D and 10.0µL of 100 µg/mL TPP into 1.00 mL of acetonitrile (ACN). (Hint: Whatis the total volume?) Separation by HPLC gave the peaks shown inthe table below. The sample was analyzed by homogenizing 5.196 g ofplant material and extracting the 2,4-D into 2.4 mL of ACN. 10.0 µLof 100 µg/mL TPP was added to the ACN extract before HPLC gave thepeak areas shown below.

Peak Areas    2,4-DTPP

Calibration Solution 28685    15865

Plant Extract    2217    5816

Calculate the concentration of 2,4-D in the plant material inunits of ng/g (ppb).

ng/g

Answer & Explanation Solved by verified expert
3.9 Ratings (784 Votes)
From the first data 10 uL 001 ml concentration of TPP in solution 100 ugml x 001 ml102 ml 098 ugml concentration of 24D in solution    See Answer
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