The following sequences show the wildtype and mutant alleles ofpart of a gene. What is the name of this type of mutation?

Wildtype DNA:       5’          AAC – AGC –CTG – CGT – ACG – GCT – CTC 3’

Wildtype protein: Asn – Ser – Leu – Arg – Thr – Ala – Leu

MutantDNA:          5’ AAC –AGC – CTG – CTT – ACG – GCT – CTC 3’

Mutant protein: Asn – Ser – Leu – Leu – Thr – Ala –Leu

Select one:

a. Frameshift mutation

b. Silent mutation

c. Missense mutation

d. Antisense mutation

What effect will a nonsense mutation in a coding triplet have onthe encoded protein?

Select one:

a. Replacement of the affected amino acid with a different aminoacid

b. No change to the amino sequence

c. Loss of the affected amino acid but retention of allremaining amino acids downstream of mutation

d. Loss of the affected amino acid and all other amino acidsdownstream of the mutation site

Once a polypeptide is produced by translation, which cellularorganelle is it transported into for correct folding?

Select one:

a. The nucleus

b. The ribosome

c. The endoplasmic reticulum

d. The Golgi

Which two techniques can be used to generate many identicalcopies of one specific DNA sequence?

Select one:

a. i) Digestion of DNA with Restriction enzymes; and ii)ligation with DNA ligase

b. i) Polymerase chain reaction; and ii) Transformation ofbacteria with a recombinant DNA plasmid

c. i) Digestion of DNA with Restriction enzymes; and ii)Transformation of bacteria with a recombinant DNA plasmid

d. i) Polymerase chain reaction; and ii) digestion of DNA withRestriction enzymes