The following sequence of 30 nucleotides corresponds to one of the two strands of a double...

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Biology

The following sequence of 30 nucleotides corresponds to one ofthe two strands of a double stranded DNA:

5’ GATGTGATCAGACCGGGTGCACTCTAATCT 3’

a) This sequence has two perfect palindromes that consist of 6base pairs each. What is the sequence of these two palindromes?

b) Show both strands of your FIRST palindrome (indicate the5’-3’ polarity)

c) Show both strands of your SECOND palindrome (indicate the5’-3’ polarity)

Assume that the two palindromes are recognized by “6-cutter”restriction enzymes, and that each enzyme creates bluntends. Assuming the 30bp DNA sequence is digested with BOTHenzymes, and that the resulting dsDNA fragments are stable (i.e.the complementary strands remain annealed):

  1. How many fragments would you expect upon digestion?
  2. How long would these fragments be (in bp)?

Answer & Explanation Solved by verified expert
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Question 1 a The two palindromes of 6 base pair are 5TGATCA3 5GTGCAC3 Palindromes are the words which read same from both the sides The order of arrangement of words is same from both side that is the reverse of word will be the same as the read from front The restriction enzymes are the specific enzymes which search the DNA strands and cuts it at the specific part Every restriction enzyme has a specific place    See Answer
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