The following is a gene sequences in human that related toovarian cancer. Design a pair of primers for PCR amplification ofthis gene using Primer 3. Print the primer 3 output, and answer thefollowing questions:

DNA sequences

•1 ttaaaagttc actggaactg gaagaaatgg atttatctgc cgtccaaattcaagaagtac 61 aaaatgtcct tcatgctatg cagaaaatct tagagtgtccgatctgtttg gaactgatca 121 aagaacctgt ttccacaaag tgtgaccacatattttgcaa attttgtatg ctgaaacttc 181 ttaaccagaa gaaagggccttcacaatgtc ctttgtgtaa gaatgagata accaaaagga 241 gcctacagggaagcacaagg tttagtcagc ttgctgaaga gctgctgaga ataatggctg 301cttttgagct tgacacggga atgcagctta caaatggttt tagtttttca aaaaagagaa361 ataattcttg tgagcgtttg aatgaggagg cgtcgatcat ccagagcgtgggctaccgga 421 accgtgtcag aaggcttccc caggtcgaac ctggaaatgccaccttgaag gacagcctag 481 gtgtccagct gtctaacctt ggaatcgtgagatcagtgaa gaaaaacagg cagacccaac 541 ctcgaaagaa atctgtctacattgaactag actctgattc ttctgaagag acagtaacta 601 agccaggtgattgcagtgtg agagaccagg aattgttaca gaccgcccct caagaagctg 661gagatgaagg caagctgcac tctgcagaag aggctgcttg tgagttttct gagggcataa721 gaaacattga acatcatcaa tgcagtgatg atttaaaccc tactgagaatcatgcaactg 781 aaaggcatcc agaaaaatgt cagagtattt ctatttcaaatgtgtgtgtg gagccatgtg 841 gcacagatgc tcatgccagc tcattacagcctgagaccag cagtttattg ctcattgaag 901 acagaatgaa tgcagaaaaggctgaattct gtaataaaag caaacagcct ggcatagcag 961 tgagccagcagagcagatgg gctgcaagta aaggaacatg taacgacagg caggttccca 1021gcactgggga aaaggtaggt ccaaacgctg actcccttag tgatagagag aagtggactc1081 acccgcaaag tctgtgccct gagaattctg gagctaccac cgatgttccttggataacac 1141 taaatagcag cgttcagaaa gttaatgagt ggttttccagaactggtgaa atgttaactt 1201 ctgacagcgc atctgccagg aggcacgagtcaaatgctga agcagctgtt gtgttggaag 1261 tttcaaacga agtggatgggggttttagtt cttcaaggaa aacagactta gtaacccccg 1321 acccccatcatactttaatg tgtaaaagtg gaagagactt ctccaaacca gtagaggata 1381atatcagtga taaaatattt gggaaatcct atcagagaaa gggaagccgc cctcacctga1441 accatgtgac tgaaattata ggcacattta ttacagaacc acagataacacaagagcagc 1501 ccttcacaaa taaattaaaa cgtaagagaa gtacatcccttcaacctgag gacttcatca 1561 agaaagcaga ttcagcaggt gttcaaaggactcctgacaa cataaatcag ggaactgacc 1621 taatggagcc aaatgagcaagcagtgagta ctaccagtaa ctgtcaggag aacaaaatag

1. What is the sequences of the primers? (5’-3’)

●

2.What is the size of amplification products

3.What is the length of your primer?

●

4.What is your GC content of your primer (GC%)?

●