The following data was generated for an isolated mammaliandihydrofolate reductase.
[7,8-dihydrofolate](mM)Â Â Â Â Â Â Â Â Â V(mol/s)Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â V (mol/s) with methotrexate
               1.1                                 333                                     169
               2.0                                 444                                     265
               5.0                                 667                                     465
A.       Graph the data withand without methotrexate on a Lineweaver-Burk plot. Clearly labelwhich line is which.
B.       CalculateKm for the enzyme in the presence and absence ofmethotrexate. Is this a competitive or non-competitiveinhibitor?
C.       Considering thatdeoxynucleotide concentrations are limiting in the cell, whateffect will addition of methotrexate have on DNA replication?Explain your reasoning.
