The following data was generated for an isolated mammalian dihydrofolate reductase. [7,8-dihydrofolate] (mM)          V (mol/s)                V (mol/s) with methotrexate                 1.1                                  333                                      169                 2.0                                  444                                      265                 5.0                                  667                                      465 A.        Graph...

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Chemistry

The following data was generated for an isolated mammaliandihydrofolate reductase.

[7,8-dihydrofolate](mM)          V(mol/s)               V (mol/s) with methotrexate

               1.1                                 333                                     169

               2.0                                 444                                     265

               5.0                                 667                                     465

A.        Graph the data withand without methotrexate on a Lineweaver-Burk plot. Clearly labelwhich line is which.

B.        CalculateKm for the enzyme in the presence and absence ofmethotrexate. Is this a competitive or non-competitiveinhibitor?

C.        Considering thatdeoxynucleotide concentrations are limiting in the cell, whateffect will addition of methotrexate have on DNA replication?Explain your reasoning.

Answer & Explanation Solved by verified expert
3.7 Ratings (577 Votes)
Theory of LineweaverBurk plota b by the theory if you    See Answer
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