The following chemicals have the indicated formula weights: Sucrose -- 342.3g/mol Potassium dihydrogen phosphate (KH2P04) -- 136.09g/mol You...

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Biology

The following chemicals have the indicated formula weights:
Sucrose -- 342.3g/mol
Potassium dihydrogen phosphate (KH2P04) -- 136.09g/mol
You also have the following solutions already made: 20% SDS, 5MNaCl and 10X TAE List the steps to make the following buffers (bespecific and show calculations):
a. 1 liter of a solution containing: 0.5 M sucrose, 0.05 M NaCl,0.02 M KH2PO4.
b. 100 ml of 10% SDS, 1M NaCl.
c. 40 ml 0.5% agarose in 1X TAE solution (calculate for bothagarose and 1X TAE).

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4.1 Ratings (809 Votes)

a Sucrose formula weight3423gmol as formula weight means total sum of the atomic weight of a particular compound thus 1 mole of sucrose has the mass corresponding to 3423gm to make 1 M 1 lt solution we need 3423 gm sucrose thus for 05M 1 lt solution we need 3423 X 05 117115gm of sucrose We already have 5M Nacl See Answer

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