The following chart show the results of cloning nuclei fromseveral cell lines (e.g. v6.5, v18.6, etc.) derived from twodifferent genetic strains of mice (F1 and 129). Note: your answersrequire no prior knowledge of these strains or the specific celllines listed)

Genetic Background	Cell Line	Passage Number	Activated Oocytes	Embryos Transferred (% Activated)	Dead (% Transferred)	Alive (% Transferred)	Survived Long Term (% Transferred)
F1	V6.5	5-6	149	21 (14%)	0	4	4 (19%)
F1	V6.5 sc84	8-9	46	6 (13%)	2	2	2 (33%)
F1	rtA2 SD-18	8-9	32	7 (22%)	0	1	1 (14%)
Total (F1)			227	34 (15%)	2	7 (21%)	7 (21%)
129	V18.6	5-6	107	19 (19%)	1	4	0
129	J1	11	145	26 (18%)	2	4	0
129high	J1	35	92	23 (25%)	10	0	0
129	LJG-13	8-9	74	8 (11%)	4	0	0
Total (129)			418	76 (18%)	17	8 (11%)	0 (0%)

1. What is the main conclusion that can be drawnbased on the results of these data with respect to success of thecloning experiments?
2. Give three possible cellintrinsic differences between v6.5 cells andLJG-13 cells that could play a role in their different results inthis assay.

For one of the three potential factors you mention above,describe a brief experimental plan to test whetherthis factor is responsible for the differences between v6.5 cellsand LJG-13 cells in this assay. Include in this plan roughly howyou would perform the experiment, what your readout would be andwhat you could conclude based on your results.