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  3. the final concentration of dntps in a pcr should b

The final concentration of dNTPs in a PCR should be 1 mM. You currently have each...

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Biology

  1. The final concentration of dNTPs in a PCR should be 1 mM. Youcurrently have each nucleotide as separate 10 mM stocks. How wouldyou made up 400 µl of a dNTP mix?
  2. You are given a 8 mM solution of a protein (MW 27,000 g/mol).Using this information fill in the blanks below to fit the unitsgiven.
  1. ____________________μmole/ml
  2. ___________________ g/L
  3. ___________________ mg/ml
  1. You order a 19-mer oligonucleotide for a PCR reaction. Themolecular weight is 5858.9 and the amount you receive is 36.7µg.You need to make up a 5µM solution. What volume of water do youdissolve your oligo in?
  2. You lypholize 1ml of a 20nM solution of a 50kDa proteindissolved in water. What is the expected weight of the driedprotein?
  3. If the average molecular mass of an amino acid is 110Da, whatwould the estimated molecular weight of a 33-mer peptide be? (inkDa)

Answer & Explanation Solved by verified expert
3.7 Ratings (341 Votes)
Either we can add 10 l of each dNTP of total 40 l and thenaddition    See Answer
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