The enzyme HIV-1 reverse transcriptae (HIV-RT) plays asignificant role for the HIV virus and is an important drug target.Assume a concentration [E] of 2.00 µM (i.e. 2.00 x 10-6 mol/l) forHIV-RT. Two potential drug molecules, D1 and D2, were identified,which form stable complexes with the HIV-RT.
The dissociation constant of the complex ED1 formed by HIV-RTand the drug D1 is 1.00 nM (i.e. 1.00 x 10-9 mol/l). Thedissociation constant of the complex ED2 formed by HIV-RT and thedrug D2 is 100 nM (i.e. 1.00 x 10-7 mol/l).
Compute the total concentration of [D1]tot that is needed tobind 90% of the HIV-RT at the given concentration [E]tot.You do NOThave to consider competition betwwen the drugs D1 and D2! They areadministered separately.
Compute the total concentration of [D2]tot that is needed tobind 90% of the HIV-RT at the given concentration [E]tot. You doNOT have to consider competition between the drugs D1 and D2! Theyare administered separately.
